Factor VIII-related antigen

ANTISERUM: DAKO (A0082). Polyclonal (rabbit) antibody.

IMMUNOGEN: Human plasma. This antibody reacts with human von Willebrand factor. Other human plasma proteins have been removed by solid-phase absorption.

REACTIVITY ACCORDING TO MANUFACTURER: Staining of endothelial cells and megakaryocytes by immunohistochemistry on human tissues.

STAINING METHODS: Paraffin sections: It is recommended proteolytic digestion. Dilution of 1/200 with PAP technique.

WORKING DILUTION: 1/800. Pretreatment with proteinase K.

METHOD: LSAB 2-PO. 30 min. RT.

CELLS/TISSUES STAINED (CANINE UNLESS SPECIFIED): Skin: Endothelial cells and plasma. Reactivity in variable numbers of neoplastic angiosarcoma cells.

REFERENCES:

Blann AD (1990) Normal levels of von Willebrand factor antigen in human body fluids. Biologicals 18:351-353.

Bukh A, Ingerslev J, Stenbjerg S et al. (1986) The multimeric structure of plasma F VIII:Rag studied by electroelution and immunoperoxidase detection. Thromb Res 43:579-584.

Cejka J (1982) Enzyme immunoassay for factor VIII-related antigen. Clin Chem 28:1356-1358.

Sehested M, Hou-Jensen K (1981) factor VIII-related antigen as an endothelial cell marker in benign and malignant diseases. Virchows Arc (Pathol Anat) 391:217-225.

Weidner N, Semple JP, Welch WR et al. (1991) Tumor angiogenesis and metastasis. Correlation in invasive breast carcinoma. New England J Med 324:1-8.

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IMAGES

Skin, dermis, dog. Hemangiosarcoma. Immunoperoxodase-DAB for factor VIII-rAg.

 

Skin, dermis, dog. Lymphangioma. Immunoperoxidase-DAB for factor VIII-rAg.